PowerPlex(R) 21 GMIDX Files Revision History

Original Release (v1.0):  12/2011
Allele sizing and panel ranges based on mean sizing values observed between 31XX and 35XX instrument platforms
2800M positive control genotype in panel file.
Stutter filter based on mean plus 3X standard deviations of observed stutter at each locus.
PowerPlex_21_Panels_IDX_v1.0
PowerPlex_21_Bins_IDX_v1.0
PowerPlex_21_Stutter_IDX_v1.0


Version 1.1 Release:  09/2012

The bin width for D12S391 alleles that are one base apart have been changed so that all bins extend 0.5 bases larger than the allele. This change has been made because some sequence variants of D12S391 alleles (above 20 repeats) migrate slightly larger than the alleles in the allelic ladder. Previously, some D12S391 bins (in Version 1.0) 0.4 bases larger than the allele.

Example of version 1.0
Marker Name	D12S391			

20	160.20	0.5	0.4	
20.1	161.21	0.5	0.5	virtual
20.3	163.22	0.5	0.4	virtual

Example of version 1.1
Marker Name	D12S391			

20	160.2	0.4	0.5	
20.1	161.21	0.4	0.5	virtual
20.3	163.22	0.5	0.5	virtual

Version 1.2 Release 09/2014
The lower value of the stutter range for Penta E and Penta D from 3.75 to 4.25.



Version 2.0 Release:  05/2015
Allele sizing and panel ranges based on mean sizing values observed between 31XX and 35XX instrument platforms using POP-4(TM) Polymer with WEN ILS 500 size standard.

Additional virtual alleles added for following loci to match virtual alleles present in bins for loci shared between PowerPlex 21 and Fusion 6C Systems:

D3S1358: 14.3, 15.1, 15.3, 16.2, 17.2, 18.1, 18.2, 18.3
D1S1656: 16.2
D16S539: 8.3, 10.3, 12.2
D18S51: 12.3, 13.1, 15.3, 16.3, 17.3, 21.1, 23.2, 24.2
D2S1338: 22.2, 29
CSF1PO: 8.3, 12.1, 13.3
Penta D: 6.4, 8.2, 11.1, 12.1, 14.4
TH01: 5.3, 6.1, 6.3, 7.1, 7.3, 9.1
vWA: 16.1, 18.2, 18.3
D21S11: 23.3, 30.1, 31.3, 32.3, 33.3, 34.3
D7S820: 5.2, 7.1, 8.2, 9.2, 11.2, 12.3, 14.1
TPOX: 10.3
D8S1179: 6, 12.3, 13.2, 13.3, 14.1, 15.1, 15.3
D12S391: 21.3, 22.3
D19S433: 6, 8.2, 11.1, 13.1, 14.1, 14.3, 16.1, 18.1
FGA: 16.2, 19.1, 21.1, 26.3, 27.3, 28.3, 29.1, 32, 51.2


The following virtual alleles have been removed:

D19S433: 20, 20.2

PowerPlex_21_Panels_IDX_v2.0
PowerPlex_21_Bins_IDX_v2.0
PowerPlex_21_Stutter_IDX_v2.0